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RPM VS. RCF “WHAT’S THE DIFFERENCE?”
RPM
- RPM – stands for “Revolutions Per Minute” and is the speed that your rotor spins in your centrifuge.
- RPM is specific to individual centrifuge brands and rotor sizes.
RCF
- RCF – stands for “Relative Centrifugal Force” and is better known as g-force. This indicates the actual force resulting from the spinning action in the centrifuge.
- It is g-force that drives sample separation.
- The following equation can be used to convert RPM to RCF for any model centrifuge.
- RCF = 0.00001118 x radius of rotation (cm) x RPM2
Note: radius of rotation is given by the distance of the furthest most point of the tube from the axis of rotation. Please refer to your centrifuge user guide for radius value.